The diagnosis of malign lymphomas is one of the most difficult areas in histopathology. Although many cases are diagnosed through histomorphological and immunohistochemical data, occasionally, the differential diagnosis between a reactive process and a malign lymphoma is difficult to determine. In these cases, the detection of clonality through molecular analysis by PCR of rearrangements of immunoglobulin (Ig) and TCR genes is an instrument of great value in the diagnosis of lymphoproliferative processes B and T. The rearrangements for Ig and TCR occur in the hypervariable regions of these genes. Each mature lymphocyte presents a specific rearrangement with a single length and sequencing in these regions. Therefore, if what is amplified is the DNA of a normal or reactive lymphoid population, the result will be multiple fragments within a given size range, with a Gaussian distribution. When amplifying DNA from a tumor and clonal process, all the resulting fragments will be identical in sequence and size, obtaining a single majority band or peak.

• It contains two mixes of multiplex reaction (Mix A and Mix B) of the V-J region of the TCRgamma gene and an internal amplification control (IC) to check the DNA quality.
• The amplification mixes are presented in mono-test format in PCR tube strips of 0.2 ml, identified in different colors.
• All PCR mixes include 5’ end labeled primers with fluorochrome 6-FAM, allowing automatic analysis of fragments by capillary electrophoresis (compatible with ABI PRISM® 310, 3100, 3130, 3130xl, 3500 and 3500xL Genetic Analyzers).
• All amplifications can be performed with a single program in the thermal cycler.
• The Hot Start II DNA Polymerase is enzyme is provided in the kit.
• Clonal and polyclonal DNA positive controls are included.